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Western Blots of phospho- and dephospho-proteins to demonstrate the selectivity of the Phospho-Specific Antibody. The Pan-Specific Antibody recognized both the dephospho-Tyrosine Hydroxylase (dephospho-TH) and the phospho-Tyrosine Hydroxylase (phospho-TH). Most importantly, the Phospho-Specific Antibody recognized only phospho-TH whereas the Dephospho-Specific Antibody reacted selectively with dephospho-TH.<br><br><b>Below:</b>:Immunohistochemical staining of retina with the Pan-Tyrosine Hydroxylase (Pan-TH) and Phospho-Specific Tyrosine Hydroxylase (Phospho-TH) Antibodies. The Pan-TH Antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the Phospho-TH Antibody selectively labels only the two amacrine cells in this light-stimulated retina example
Western Blots of phospho- and dephospho-proteins to demonstrate the selectivity of the Phospho-Specific Antibody. The Pan-Specific Antibody recognized both the dephospho-Tyrosine Hydroxylase (dephospho-TH) and the phospho-Tyrosine Hydroxylase (phospho-TH). Most importantly, the Phospho-Specific Antibody recognized only phospho-TH whereas the Dephospho-Specific Antibody reacted selectively with dephospho-TH.

Below::Immunohistochemical staining of retina with the Pan-Tyrosine Hydroxylase (Pan-TH) and Phospho-Specific Tyrosine Hydroxylase (Phospho-TH) Antibodies. The Pan-TH Antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the Phospho-TH Antibody selectively labels only the two amacrine cells in this light-stimulated retina example

Tyrosine Hydroxylase (phospho Ser40) Antibody

CATALOG NUMBER: XPS-1033

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Pig
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Pig
Immunogen:
TH (Ser40) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser40 of rat tyrosine hydroxylase (TH).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB). Human, porcine, quail, mouse, and non-human primate have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC and WB.
Specificity:
Tyrosine Hydroxylase antibody is specific for the 60k rat TH phosphorylated at Ser40 in lysates of PC-12 Cells stimulated by Okadaic Acid (1 μM for 60 minutes). Some higher molecular weight bands may be detected depending upon the brain region being studied, protein loads, and the detection methods used. See cited reference.
Predicted Molecular Weight:
60
Purification:
Affinity Purified
Clonality:
Polyclonal
physical-state:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Ncbi Official Symbol:
Th
Protein Accession Number:
P04177
Protein Gi Number:
136577
Ncbi Gene Id Number:
25085
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons. We raised this polyclonal antibody against a peptide representing the sequence around Ser40 in rat TH. This antibody is suitable for most immunochemical applications in a variety of mammalian and some non-mammalian species.
Background Reference 1:
Witkovsky, P., Gabriel, R., Haycock, J. W., and Meller, E., "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina," J. Chem. Neuroanat., 19 (2000) 105 - 116.
Background Reference 2:
Haycock, J. W., Lew, J. Y., Garcia-Espana, A., Lee, K. Y., Harada, K., Meller, E., and Goldstein, M., "Role of serine-19 phosphorylation in regulating tyrosine hydroxylase studied with site- and phosphospecific antibodies and site-directed mutagenesis," J. Neurochem., 71 (1998) 1670-1675.
Background Reference 3:
Xu, Z.-Q., Lew, J. Y., Harada, K., Aman, K., Goldstein, M., Deutch, A., Haycock, J. W., and Hokfelt, T., "Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies," Neuroscience, 82 (1998) 727-738.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1033

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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