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Western blot analysis of extracts of various cell lines, using U2AF2 antibody (18-399) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using U2AF2 antibody (18-399). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of SW620 cells using 1ug U2AF2 antibody (18-399). Western blot was performed from the immunoprecipitate using U2AF2 antibody (18-399) at a dilition of 1:500.
Western blot analysis of extracts of various cell lines, using U2AF2 antibody (18-399) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

U2AF2 Antibody

CATALOG NUMBER: 18-399

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 192-471 of human U2AF2 (NP_001012496.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
IP: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
SW620
Positive Control 3:
Mouse spleen
Predicted Molecular Weight:
Observed: 54kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
U2AF2
Additional Names:
U2AF2, U2AF65
Ncbi Gene Id Number:
11338
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-399

List Size:
100 uL

List Price:

$436.00

Datasheet
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