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Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.

U2AF2 Antibody

CATALOG NUMBER: 29-247

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human U2AF2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
U2AF2 antibody can be used for detection of U2AF2 by ELISA at 1:62500. U2AF2 antibody can be used for detection of U2AF2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
52 kDa, 54 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store U2AF2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
U2AF2
Additional Names:
U2AF2, U2AF65
Protein Accession Number:
NP_001012496
Protein Gi Number:
60279268
Ncbi Gene Id Number:
11338
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF2 is the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly.U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
Background Reference 1:
Sickmier, E.A., Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 62 (PT.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-247

List Size:
100 ul

List Price:

$473.00

Datasheet
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