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Western blot analysis in Hela cell line lysates (35ug/lane).
UBA52 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Hela cell line lysates (35ug/lane).

UBA52 Antibody

CATALOG NUMBER: 55-070

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Monkey, Mouse, Pig, Rat
Immunogen:
This UBA52 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-128 amino acids from the C-terminal region of human UBA52.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
15 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBA52
Additional Names:
Ubiquitin-60S ribosomal protein L40, CEP52, Ubiquitin A-52 residue ribosomal protein fusion product 1, Ubiquitin, 60S ribosomal protein L40, UBA52, UBCEP2
Protein Accession Number:
P62987
Protein Gi Number:
302393718
Ncbi Gene Id Number:
7311
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ubiquitin is a highly conserved nuclear and cytoplasmicprotein that has a major role in targeting cellular proteins fordegradation by the 26S proteosome. It is also involved in themaintenance of chromatin structure, the regulation of geneexpression, and the stress response. Ubiquitin is synthesized as aprecursor protein consisting of either polyubiquitin chains or asingle ubiquitin moiety fused to an unrelated protein. This geneencodes a fusion protein consisting of ubiquitin at the N terminusand ribosomal protein L40 at the C terminus, a C-terminal extensionprotein (CEP). Multiple processed pseudogenes derived from thisgene are present in the genome.
Background Reference 1:
Memet, S. Biochem. Pharmacol. 72(9):1180-1195(2006)
Background Reference 2:
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
Background Reference 3:
Foster, L.J., et al. J. Proteome Res. 5(1):64-75(2006)
Background Reference 4:
Hu, Y., et al. Mol. Cell Proteomics 4(12):2000-2009(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-070

List Size:
400 ul

List Price:

$495.00

Datasheet
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