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Western blot analysis of extracts of various cell lines, using UBA6 antibody (22-995) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human kidney using UBA6 antibody (22-995) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using UBA6 antibody (22-995). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug UBA6 antibody (22-995). Western blot was performed from the immunoprecipitate using UBA6 antibody (22-995) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using UBA6 antibody (22-995) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

UBA6 Antibody

CATALOG NUMBER: 22-995

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human UBA6 (NP_060697.4).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:100
Positive Control 1:
U-251MG
Positive Control 2:
SKOV3
Positive Control 3:
293T
Positive Control 4:
Mouse brain
Predicted Molecular Weight:
Observed: 59kDa, 118kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
UBA6
Additional Names:
UBA6, FLJ10808, Monocyte protein 4, MOP-4, Ubiquitin-activating enzyme 6, E1-L2, MOP4, UBE1L2
Ncbi Gene Id Number:
55236
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-995

List Size:
100 uL

List Price:

$436.00

Datasheet
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