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Western blot analysis in K562 cell line lysates (35ug/lane).
UBAC1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

UBAC1 Antibody

CATALOG NUMBER: 61-898

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This UBAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-292 amino acids from the C-terminal region of human UBAC1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBAC1
Additional Names:
Ubiquitin-associated domain-containing protein 1, UBA domain-containing protein 1, E3 ubiquitin-protein ligase subunit KPC2, Glialblastoma cell differentiation-related protein 1, Kip1 ubiquitination-promoting complex protein 2, UBAC1, GBDR1, KPC2, UBADC1
Protein Accession Number:
Q9BSL1
Protein Gi Number:
74752306
Ncbi Gene Id Number:
10422
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
UBAC1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein has two RRM domains that bind to RNAs.
Background Reference 1:
Walker, L.C., et al. Breast Cancer Res. Treat. 112(2):229-236(2008)
Background Reference 2:
Wu, Y.Y., et al. Cell Biochem. Funct. 26(4):467-477(2008)
Background Reference 3:
Reboll, M.R., et al. RNA 13(8):1328-1340(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-898

List Size:
400 ul

List Price:

$495.00

Datasheet
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