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42-848 (1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-848 (3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-848 (1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UBASH3A Antibody

CATALOG NUMBER: 42-848

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DSGIRISSVFASP
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in Human Spleen lysates (calculated MW of 69.8kDa according to NP_001001895.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Breast shows perinuclear staining in the epithelial cells of the lubules. Recommended concentration, 3-5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_061834.1; NP_001001895.1).
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
UBASH3A
Additional Names:
UBASH3A, ubiquitin associated and SH3 domain containing A, CLIP4, STS-2, TULA, OTTHUMP00000109368, T-cell ubiquitin ligand protein, cbl-interacting protein 4, suppressor of T-cell receptor signaling 2, ubiquitin-associated and SH3 domain-containing protein A
Protein Accession Number:
NP_061834.1,NP_001001895.1
Protein Gi Number:
9507221
Ncbi Gene Id Number:
53347

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-848

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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