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Antibody is used in Western blot to detect UBE1 in mouse brain tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis in HL-60 cell line lysates (35ug/lane).
Western blot analysis of UBE1C using rabbit polyclonal UBE1C Antibody (S430) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE1C gene.
Antibody is used in Western blot to detect UBE1 in mouse brain tissue lysate.

UBE1C Antibody

CATALOG NUMBER: 55-304

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Rat
Immunogen:
This UBE1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 415-445 amino acids from the C-terminal region of human UBE1C.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBA3
Additional Names:
NEDD8-activating enzyme E1 catalytic subunit, 632-, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C, Ubiquitin-like modifier-activating enzyme 3, Ubiquitin-activating enzyme 3, UBA3, UBE1C
Protein Accession Number:
Q8TBC4
Protein Gi Number:
83305811
Ncbi Gene Id Number:
9039
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Background Reference 1:
Bohnsack, R.N., et al., J. Biol. Chem. 278(29):26823-26830 (2003).
Background Reference 2:
Walden, H., et al., Nature 422(6929):330-334 (2003).
Background Reference 3:
Gong, L., et al., J. Biol. Chem. 274(17):12036-12042 (1999).
Background Reference 4:
Gubin, A.N., et al., Genomics 59(2):168-177 (1999).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-304

List Size:
400 ul

List Price:

$495.00

Datasheet
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