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Western blot analysis in Hela cell line lysates (35ug/lane).
Western blot analysis of UBE2D3 using rabbit polyclonal UBE2D3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2D3 gene.
Western blot analysis in Hela cell line lysates (35ug/lane).

UBE2D3 Antibody

CATALOG NUMBER: 58-731

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Pig, Bovine, Monkey, Mouse, Rat
Immunogen:
This UBE2D3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-136 amino acids from the C-terminal region of human UBE2D3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
17 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBE2D3
Additional Names:
Ubiquitin-conjugating enzyme E2 D3, Ubiquitin carrier protein D3, Ubiquitin-conjugating enzyme E2(17)KB 3, Ubiquitin-conjugating enzyme E2-17 kDa 3, Ubiquitin-protein ligase D3, UBE2D3, UBC5C, UBCH5C
Protein Accession Number:
P61077
Protein Gi Number:
46577654
Ncbi Gene Id Number:
7323
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The modification of proteins with ubiquitin is animportant cellular mechanism for targeting abnormal or short-livedproteins for degradation. Ubiquitination involves at least threeclasses of enzymes: ubiquitin-activating enzymes, or E1s,ubiquitin-conjugating enzymes, or E2s, and ubiquitin-proteinligases, or E3s. This gene encodes a member of the E2ubiquitin-conjugating enzyme family. This enzyme functions in theubiquitination of the tumor-suppressor protein p53, which isinduced by an E3 ubiquitin-protein ligase. Multiple splicedtranscript variants have been found for this gene, but thefull-length nature of some variants has not been determined.
Background Reference 1:
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Background Reference 2:
Wu, K., et al. Mol. Cell 37(6):784-796(2010)
Background Reference 3:
Vina-Vilaseca, A., et al. J. Biol. Chem. 285(10):7645-7656(2010)
Background Reference 4:
Markson, G., et al. Genome Res. 19(10):1905-1911(2009)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-731

List Size:
400 ul

List Price:

$495.00

Datasheet
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