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Western blot analysis of lysate from Jurkat cell line, using UBE2D4 Antibody at 1:1000 at each lane.
Western blot analysis of lysate from Jurkat cell line, using UBE2D4 Antibody at 1:1000 at each lane.

UBE2D4 Antibody

CATALOG NUMBER: 60-294

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus
Immunogen:
This UBE2D4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human UBE2D4.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
17 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBE2D4
Additional Names:
Ubiquitin-conjugating enzyme E2 D4, HBUCE1, Ubiquitin carrier protein D4, Ubiquitin-protein ligase D4, UBE2D4, UBCH5D
Protein Accession Number:
Q9Y2X8
Protein Gi Number:
74753478
Ncbi Gene Id Number:
51619
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.
Background Reference 1:
Markson, G., et al. Genome Res. 19(10):1905-1911(2009)
Background Reference 2:
van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :
Background Reference 3:
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Background Reference 4:
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-294

List Size:
400 ul

List Price:

$495.00

Datasheet
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