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Western blot analysis in mouse Neuro-2a,Jurkat,Ramos cell line lysates (35ug/lane).
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse Neuro-2a,Jurkat,Ramos cell line lysates (35ug/lane).

UBE2E2 Antibody

CATALOG NUMBER: 57-295

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This UBE2E2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-43 amino acids from the N-terminal region of human UBE2E2.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBE2E2
Additional Names:
Ubiquitin-conjugating enzyme E2 E2, UbcH8, Ubiquitin carrier protein E2, Ubiquitin-protein ligase E2, UBE2E2, UBCH8
Protein Accession Number:
Q96LR5
Protein Gi Number:
47606201
Ncbi Gene Id Number:
7325
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination.
Background Reference 1:
Yamauchi, T., et al. Nat. Genet. 42(10):864-868(2010)
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)
Background Reference 4:
Ng, D., et al. Int. J. Cancer 123(7):1610-1615(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-295

List Size:
400 ul

List Price:

$495.00

Datasheet
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