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Western blot analysis of UBE2H using rabbit polyclonal UBE2H Antibody (S167) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2H gene.
Western blot analysis of UBE2H using rabbit polyclonal UBE2H Antibody (S167) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2H gene.

UBE2H Antibody

CATALOG NUMBER: 61-084

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This UBE2H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-183 amino acids from the C-terminal region of human UBE2H.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBE2H
Additional Names:
Ubiquitin-conjugating enzyme E2 H, UbcH2, Ubiquitin carrier protein H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase H, UBE2H
Protein Accession Number:
P62256
Protein Gi Number:
51338683
Ncbi Gene Id Number:
7328
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2H is a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs, which indicates that this enzyme is highly conserved in eukaryotes.
Background Reference 1:
Hayashida, S., et al., Genomics 66(2):221-225 (2000).
Background Reference 2:
Kaiser, P., et al., J. Biol. Chem. 269(12):8797-8802 (1994).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-084

List Size:
400 ul

List Price:

$495.00

Datasheet
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