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Antibody used in WB on Human Jurkat 2.5 ug/ml.
Antibody used in WB on Human Jurkat 2.5 ug/ml.

UBE2I Antibody

CATALOG NUMBER: 29-821

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2I.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
UBE2I antibody can be used for detection of UBE2I by ELISA at 1:312500. UBE2I antibody can be used for detection of UBE2I by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
17 kDa, 18 kDa, 18 kDa, 18 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store UBE2I antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
UBE2I
Additional Names:
UBE2I, C358B7.1, P18, UBC9
Protein Accession Number:
NP_003336
Protein Gi Number:
4507785
Ncbi Gene Id Number:
7329
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2I is a member of the E2 ubiquitin-conjugating enzyme family.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
Background Reference 1:
Zhu, S., (2006) Exp. Cell Res. 312 (7), 1042-1049.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-821

List Size:
100 ul

List Price:

$473.00

Datasheet
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