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Immunohistochemical analysis of paraffin-embedded H.kidney section using UBE2L3 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis in K562,U87-MG,mouse NIH/3T3,rat PC-12 cell line and mouse testis,rat testis tissue lysates (35ug/lane).
Immunohistochemical analysis of paraffin-embedded H.kidney section using UBE2L3 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

UBE2L3 Antibody

CATALOG NUMBER: 60-766

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This UBE2L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the C-terminal region of human UBE2L3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
18 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBE2L3
Additional Names:
Ubiquitin-conjugating enzyme E2 L3, L-UBC, UbcH7, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3, UBE2L3, UBCE7, UBCH7
Protein Accession Number:
P68036
Protein Gi Number:
54039805
Ncbi Gene Id Number:
7332
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The modification of proteins with ubiquitin is animportant cellular mechanism for targeting abnormal or short-livedproteins for degradation. Ubiquitination involves at least threeclasses of enzymes: ubiquitin-activating enzymes (E1s),ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases(E3s). This gene encodes a member of the E2 ubiquitin-conjugatingenzyme family. This enzyme is demonstrated to participate in theubiquitination of p53, c-Fos, and the NF-kB precursor p105 invitro. Several alternatively spliced transcript variants have beenfound for this gene.
Background Reference 1:
Fransen, K., et al. Hum. Mol. Genet. 19(17):3482-3488(2010)
Background Reference 2:
Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)
Background Reference 3:
Kamatani, Y., et al. Nat. Genet. 42(3):210-215(2010)
Background Reference 4:
Purbeck, C., et al. Biochemistry 49(7):1361-1363(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-766

List Size:
400 ul

List Price:

$495.00

Datasheet
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