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Antibody used in WB on Human NT-2, mouse brain at 2 ug/ml.
Antibody used in WB on r-UBE2N at: 1:500 (Lanes: 1: 40ng HIS-UBE2D1 protein, 2: 40ng HIS-UBE2D2 protein, 3: 40ng HIS-UBE2D3 proteinm, 4: 40ng HIS-UBE2D4 protein, 5: 40ng HIS-UBE2E1 protein, 6: 40ng HIS-UBE2E2 protein, 7: 40ng HIS-UBE2E3 protein, 8: 40ng HIS-UBE2K protein, 9: 40ng HIS-UBE2L3 protein, 10: 40ng HIS-UBE2N protein, 11: 40ng HIS-UBE2V1 protein, 12: 40ng HIS-UBE2V2 protein.).
Antibody used in IHC on Mouse brainstem at 1:500.
Antibody used in WB on Human Jurkat cells at 2.5 ug/ml.
Antibody used in WB on Human NT-2, mouse brain at 2 ug/ml.

UBE2N Antibody

CATALOG NUMBER: 27-794

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Drosophila, Human, Mouse, Rat, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Drosophila, Human, Mouse, Rat, Zebrafish
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2N.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
UBE2N antibody can be used for detection of UBE2N by ELISA at 1:1562500. UBE2N antibody can be used for detection of UBE2N by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
17 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store UBE2N antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
UBE2N
Additional Names:
UBE2N, MGC131857, MGC8489, UBC13, UbcH-ben, UbcH13, HEL-S-71
Protein Accession Number:
NP_003339
Protein Gi Number:
4507793
Ncbi Gene Id Number:
7334
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
UBE2N encodes a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
Background Reference 1:
Zhang, M., (2005) Mol. Cell 20 (4), 525-538.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-794

List Size:
100 ul

List Price:

$473.00

Out of stock

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