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Western blot analysis of extracts of various cell lines, using UBE2R2 antibody (22-897) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse cancer using UBE2R2 antibody (22-897) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using UBE2R2 antibody (22-897).
Western blot analysis of extracts of various cell lines, using UBE2R2 antibody (22-897) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

UBE2R2 Antibody

CATALOG NUMBER: 22-897

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-238 of human UBE2R2 (NP_060281.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
MCF7
Positive Control 3:
Mouse liver
Positive Control 4:
Mouse kidney
Positive Control 5:
Mouse heart
Positive Control 6:
Rat brain
Predicted Molecular Weight:
Observed: 27kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
UBE2R2
Additional Names:
ubiquitin-conjugating enzyme UBC3B, ubiquitin-conjugating enzyme E2R 2, MGC10481, FLJ20419, CDC34B, UBC3B, UBE2R2
Ncbi Gene Id Number:
54926
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-897

List Size:
100 uL

List Price:

$436.00

Datasheet
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