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Western blot analysis of UBE2T using rabbit polyclonal UBE2T Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2T gene.
Western blot analysis of UBE2T using rabbit polyclonal UBE2T Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2T gene.

UBE2T Antibody

CATALOG NUMBER: 59-158

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This UBE2T antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-154 amino acids from the C-terminal region of human UBE2T.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
23 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBE2T
Additional Names:
Ubiquitin-conjugating enzyme E2 T, Cell proliferation-inducing gene 50 protein, Ubiquitin carrier protein T, Ubiquitin-protein ligase T, UBE2T
Protein Accession Number:
Q9NPD8
Protein Gi Number:
73622065
Ncbi Gene Id Number:
29089
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The covalent conjugation of ubiquitin to proteinsregulates diverse cellular pathways and proteins. Ubiquitin istransferred to a target protein through a concerted action of aubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme(E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al.,2006 [PubMed 16916645]).
Background Reference 1:
Ueki, T., et al. Cancer Res. 69(22):8752-8760(2009)
Background Reference 2:
Longerich, S., et al. J. Biol. Chem. 284(35):23182-23186(2009)
Background Reference 3:
Alpi, A.F., et al. Mol. Cell 32(6):767-777(2008)
Background Reference 4:
Hao, J., et al. Tumour Biol. 29(3):195-203(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-158

List Size:
400 ul

List Price:

$495.00

Datasheet
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