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Western blot analysis of extracts of various cell lines, using UBE2U antibody (23-541) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human liver using UBE2U antibody (23-541) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using UBE2U antibody (23-541) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using UBE2U antibody (23-541) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

UBE2U Antibody

CATALOG NUMBER: 23-541

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human UBE2U (NP_689702.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
U-251MG
Positive Control 2:
LO2
Positive Control 3:
NCI-H460
Predicted Molecular Weight:
Observed: 42kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
UBE2U
Additional Names:
ubiquitin-conjugating enzyme E2 U, E2 ubiquitin-conjugating enzyme U, testicular tissue protein Li 216, ubiquitin carrier protein U, ubiquitin conjugating enzyme E2 U (putative), ubiquitin conjugating enzyme E2U (putative), ubiquitin-protein ligase U
Ncbi Gene Id Number:
148581
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-541

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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