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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Western blot analysis in mouse liver tissue lysates (35ug/lane).
UBE2W Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

UBE2W Antibody

CATALOG NUMBER: 57-153

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Bovine, Rat
Immunogen:
This UBE2W antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-133 amino acids from the C-terminal region of human UBE2W.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
17 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBE2W
Additional Names:
Ubiquitin-conjugating enzyme E2 W, N-terminus-conjugating E2, Ubiquitin carrier protein W, Ubiquitin-conjugating enzyme 16, UBC-16, Ubiquitin-protein ligase W, UBE2W, UBC16
Protein Accession Number:
Q96B02
Protein Gi Number:
74751754
Ncbi Gene Id Number:
55284
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
UBE2W accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and 'Lys-11'-linked polyubiquitination.
Background Reference 1:
Markson, G., et al. Genome Res. 19(10):1905-1911(2009)
Background Reference 2:
van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :
Background Reference 3:
Yin, G., et al. Front. Biosci. 11, 1500-1507 (2006) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-153

List Size:
400 ul

List Price:

$495.00

Datasheet
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