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Western Blot at 1:1000 dilution Lane 1: Caco2 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
UBE4A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution Lane 1: Caco2 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

UBE4A Antibody

CATALOG NUMBER: 57-907

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This UBE4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451-479 amino acids from the Central region of human UBE4A.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
123 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBE4A
Additional Names:
Ubiquitin conjugation factor E4 A, 632-, UBE4A (HGNC:12499)
Protein Accession Number:
Q14139
Ncbi Gene Id Number:
9354
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The modification of proteins with ubiquitin is animportant cellular mechanism for targeting abnormal or short-livedproteins for degradation. Ubiquitination involves at least threeclasses of enzymes: ubiquitin-activating enzymes, or E1s,ubiquitin-conjugating enzymes, or E2s, and ubiquitin-proteinligases, or E3s. This gene encodes an additional conjugationfactor, E4, which is involved in multiubiquitin chain assembly.
Background Reference 1:
Shi, D., et al. Proc. Natl. Acad. Sci. U.S.A. 106(38):16275-16280(2009)
Background Reference 2:
Sakiyama, T., et al. Inflamm. Bowel Dis. 14(3):310-317(2008)
Background Reference 3:
Yang, Y., et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 23(6):504-506(2007)
Background Reference 4:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-907

List Size:
400 ul

List Price:

$495.00

Datasheet
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