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Western blot analysis of anti-Ubiquitin Pab in HeLa cell lysate. Ubiquitin was detected using purified Pab.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Characterization of FOXP2 Isoforms. FOXP2.10t was detected with an antibody to the N-terminal XpressTM tag or FOXP2 (green) and counterstained with antibodies to the aggresome marker ubiquitin (red). Nuclei are marked by DAPI staining (blue). Ubiquitin co-localizes with FOXP2.10t aggregates suggesting that these cellular bodies represent aggresomes.
Western blot analysis of anti-Ubiquitin Pab in HeLa cell lysate. Ubiquitin was detected using purified Pab.

Ubiquitin Antibody

CATALOG NUMBER: 56-332

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Chicken, Yeast, Xenopus, Bovine, C. Elegans, Drosophila
Immunogen:
This Ubiquitin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-66 amino acids of human Ubiquitin.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
77 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBC
Additional Names:
Polyubiquitin-C, Ubiquitin, UBC
Protein Accession Number:
P0CG48
Protein Gi Number:
391358178
Ncbi Gene Id Number:
7316
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.
Background Reference 1:
Chan, Y.L., et al., Biochem. Biophys. Res. Commun. 215(2):682-690 (1995).
Background Reference 2:
Cook, W.J., et al., J. Mol. Biol. 236(2):601-609 (1994).
Background Reference 3:
Hubbard, M.J., et al., Biochim. Biophys. Acta 1200(2):191-196 (1994).
Background Reference 4:
Wajih, N., et al., Protein Seq. Data Anal. 5(1):31-32 (1992).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-332

List Size:
400 ul

List Price:

$495.00

Datasheet
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