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Western blot analysis of extracts of HeLa cells, using UBR4 antibody (14-306) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of HeLa cells, using UBR4 antibody (14-306) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

UBR4 Antibody

CATALOG NUMBER: 14-306

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 4884-5183 of human UBR4 (NP_065816.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:1000
Positive Control 1:
HeLa
Predicted Molecular Weight:
Observed: 600kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
UBR4
Additional Names:
UBR4, zinc finger, UBR1 type 1, retinoblastoma-associated factor 600-like protein, retinoblastoma-associated factor 600, p600, ZUBR1, RBAF600, KIAA1307, KIAA0462, FLJ41863, ubiquitin protein ligase E3 component n-recognin 4, UBR4
Ncbi Gene Id Number:
23352
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition, this protein is a target of the human papillomavirus type 16 E7 oncoprotein.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-306

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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