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Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis in K562 cell line lysates (35ug/lane).

UBTF Antibody

CATALOG NUMBER: 56-496

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This UBTF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 631-660 amino acids from the C-terminal region of human UBTF.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
89 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBTF
Additional Names:
Nucleolar transcription factor 1, Autoantigen NOR-90, Upstream-binding factor 1, UBF-1, UBTF, UBF, UBF1
Protein Accession Number:
P17480
Protein Gi Number:
136652
Ncbi Gene Id Number:
7343
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Upstream binding factor (UBF) is a transcription factorrequired for expression of the 18S, 5.8S, and 28S ribosomal RNAs,along with SL1 (a complex of TBP (MIM 600075) and multipleTBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBFis a nucleolar phosphoprotein with both DNA binding andtransactivation domains. Sequence-specific DNA binding to the coreand upstream control elements of the human rRNA promoter ismediated through several HMG boxes (Jantzen et al., 1990 [PubMed2330041]).
Background Reference 1:
Hisaoka, M., et al. Mol. Cell. Biol. 30(20):4952-4964(2010)
Background Reference 2:
Liu, Z., et al. Proteins 77(2):432-447(2009)
Background Reference 3:
Lin, C.I., et al. Biochem. Biophys. Res. Commun. 386(2):396-401(2009)
Background Reference 4:
Grob, A., et al. J. Cell. Sci. 122 (PT 4), 489-498 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-496

List Size:
400 ul

List Price:

$495.00

Datasheet
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