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IHC staining of human brain with PGP9.5 antibody (31A3).
Western blot of human, mouse and rat brain lysate using PGP9.5 antibody (31A3).
IHC staining of human brain with PGP9.5 antibody (31A3).

UchL1 Antibody [31A3]

CATALOG NUMBER: 33-221

Clonality:
Monoclonal
Tested Applications:
IHC, WB
Host Species:
Mouse
Species Reactivity:
Bovine, Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Bovine, Human, Mouse, Pig, Rat
Immunogen:
Native protein from brain was used as the immunogen for this PGP9.5 antibody.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PGP9.5 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
UCHL1
Additional Names:
Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, 6---, Neuron cytoplasmic protein 95, PGP 95, PGP95, Ubiquitin thioesterase L1, UCHL1
Ncbi Gene Id Number:
7345
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This antibody specifically reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. PGP9.5 antibody has been used to show the protein to be a useful prognostic indicator of colorectal, pancreatic and gall bladder cancer. A mutation in the gene is believed to cause a form of Parkinson's disease.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-221

List Size:
100 ug

List Price:

$626.00

Datasheet
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