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Antibody used in WB on Human MCF7 at 0.2-1 ug/ml.
Antibody used in IHC on Human Placenta.
Antibody used in WB on Zebrafish skin, liver at 1:1000 (Lane 1: 30ug Zebrafish skin lysate Lane 2: 30ug Zebrafish liver lysate ).
Antibody used in WB on Human MCF7 at 0.2-1 ug/ml.

UCHL3 Antibody

CATALOG NUMBER: 27-209

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UCHL3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
UCHL3 antibody can be used for detection of UCHL3 by ELISA at 1:1562500. UCHL3 antibody can be used for detection of UCHL3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1219 - MCF7 Cell Lysate
Predicted Molecular Weight:
25 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store UCHL3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
UCHL3
Additional Names:
UCHL3, UCH-L3
Protein Accession Number:
NP_005993
Protein Gi Number:
5174741
Ncbi Gene Id Number:
7347
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
UCHL3 is an ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8.
Background Reference 1:
Lim, J., (2006) Cell 125 (4), 801-814.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-209

List Size:
100 ul

List Price:

$519.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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