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Western blot analysis of extracts of NCCIT cells, using UCHL5 antibody (13-853).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of NCCIT cells, using UCHL5 antibody (13-853).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

UCHL5 Antibody

CATALOG NUMBER: 13-853

Clonality:
Polyclonal
Tested Applications:
Flow, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
Recombinant protein of human UCHL5
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:500 - 1:1000
IP: 1:50 - 1:100
Flow: 1:50 - 1:100
Positive Control 1:
NCCIT
Predicted Molecular Weight:
Observed: 38kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
UCHL5
Additional Names:
Ubiquitin carboxyl-terminal hydrolase isozyme L5, UCH-L5, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin thioesterase L5, UCHL5, UCH37
Ncbi Gene Id Number:
51377
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-853

List Size:
50 uL

List Price:

$365.00

Datasheet
Shipping Info

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