Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Antibody is used in Western blot to detect UCK in HepG2 cell lysate (Lane 1) and mouse cerebellum tissue lysate (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

UCK Antibody

CATALOG NUMBER: 63-411

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Pig
Immunogen:
This UCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132-162 amino acids from the C-terminal region of human UCK.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CMPK1
Additional Names:
UMP-CMP kinase {ECO:0000255, HAMAP-Rule:MF_03172}, 27414 {ECO:0000255, HAMAP-Rule:MF_03172}, Deoxycytidylate kinase {ECO:0000255, HAMAP-Rule:MF_03172}, CK {ECO:0000255, HAMAP-Rule:MF_03172}, dCMP kinase {ECO:0000255, HAMAP-Rule:MF_03172}, Nucleoside-diphosphate kinase {ECO:0000255, HAMAP-Rule:MF_03172}, 2746 {ECO:0000255, HAMAP-Rule:MF_03172}, Uridine monophosphate/cytidine monophosphate kinase {ECO:0000255, HAMAP-Rule:MF_03172}, UMP/CMP kinase {ECO:0000255, HAMAP-Rule:MF_03172}, UMP/CMPK {ECO:0000255, HAMAP-Rule:MF_03172}, CMPK1 {ECO:0000255, HAMAP-Rule:MF_03172}
Protein Accession Number:
P30085
Protein Gi Number:
12644008
Ncbi Gene Id Number:
51727
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
UCK1 (Uridine-cytidine kinase 1) phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. This enzyme does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Of note, UCK1 is able to use either ATP or GTP as a phosphate donor. UCK1 also possesses the ability to phosphorylate a number of cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine.
Background Reference 1:
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Background Reference 2:
Hu, R.M., et al., Proc. Natl. Acad. Sci. U.S.A. 97(17):9543-9548 (2000).
Background Reference 3:
Hughes, G.J., et al., Electrophoresis 14(11):1216-1222 (1993).
Background Reference 4:
Hochstrasser, D.F., et al., Electrophoresis 13(12):992-1001 (1992).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-411

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369