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46-548 (1ug/ml) staining of Rat Adipose (A) and (0.5ug/ml) Skeletal Muscle (B) lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.
46-548 (1ug/ml) staining of Rat Adipose (A) and (0.5ug/ml) Skeletal Muscle (B) lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.

UCP1 Antibody

CATALOG NUMBER: 46-548

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-EQLKRELSKSRQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Rat Adipose tissue and Skeletal Muscle lysates (calculated MW of 33.2kDa according to Rat NP_036814.1). Recommended concentration: 0.5-1 ug/ml. . Primary incubation 1 hour at room temperature.
Positive Control 1:
Cat. No. XBL-11048 - Human Adipose Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
UCP1
Additional Names:
UCP1, uncoupling protein 1 (mitochondrial, proton carrier), HGNC:12517, SLC25A7, UCP, mitochondrial brown fat uncoupling protein, uncoupling protein 1
Protein Accession Number:
NP_068605.1
Protein Gi Number:
11225256
Ncbi Gene Id Number:
7350
Background Reference 1:
Ishigaki Y, Katagiri H, Yamada T, Ogihara T, Imai J, Uno K, Hasegawa Y, Gao J, Ishihara H, Shimosegawa T, Sakoda H, Asano T, Oka Y. Dissipating excess energy stored in the liver is a potential treatment strategy for diabetes associated with obesity. Diabetes. 2005 Feb;54(2):322-32.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-548

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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