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Western blot analysis of extracts of various cell lines, using UGDH Antibody (14-260) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using UGDH antibody (14-260) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using UGDH antibody (14-260) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using UGDH antibody (14-260). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using UGDH Antibody (14-260) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

UGDH Antibody

CATALOG NUMBER: 14-260

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 165-494 of human UGDH (NP_003350.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
THP-1
Positive Control 3:
22Rv1
Positive Control 4:
ES-2
Positive Control 5:
U-937
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 56kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
UGDH
Additional Names:
UDP-glucose 6-dehydrogenase, UDP-Glc dehydrogenase, UDP-GlcDH, UDPGDH, UGDH
Ncbi Gene Id Number:
7358
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-260

List Size:
100 uL

List Price:

$436.00

Datasheet
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