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MCF7 overexpressing Human UGT8 and probed with 42-832 (mock transfection in first lane). Primary incubation (1ug/ml) was overnight at 4C. Data provided by Tomasz Owczarek, Wroclaw University, Poland. Detected by chemiluminescence.
MCF7 overexpressing Human UGT8 and probed with 42-832 (mock transfection in first lane). Primary incubation (1ug/ml) was overnight at 4C. Data provided by Tomasz Owczarek, Wroclaw University, Poland. Detected by chemiluminescence.

UGT8 Antibody

CATALOG NUMBER: 42-832

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NGKYKRNGHIK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:In transfected MCF7 transiently expressing Human UGT8 a band of approx. 65kDa is observed. This band is not observed in the non-transfected MCF7. The calculated molecular size is 61.5kDa according to NP_003351.2. Recommended concentration, 1-3ug/ml.Preliminary experiments gave an approx 50kDa band in Human -Brain lysates and 48KDa in Rat Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 61.5kDa according to NP_003351.2. The observed band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
Reported variants represent identical protein: NP_001121646.1, NP_003351.2.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 1461 - Rat Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
UGT8
Additional Names:
UGT8, UDP glycosyltransferase 8, CGT, UGT4, 2-hydroxyacylsphingosine 1-beta-galactosyltransferase, UDP-galactose ceramide galactosyltransferase, UDP-galactose-ceramide galactosyltransferase, ceramide UDP-galactosyltransferase, cerebroside synthase, uridine diphosphate glycosyltransferase 8
Protein Accession Number:
NP_003351.2
Protein Gi Number:
40254471
Ncbi Gene Id Number:
7368

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-832

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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