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Western Blot at 1:2000 dilution + mouse cerebellum lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human heart lysates Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).
UGT8 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:2000 dilution + mouse cerebellum lysate Lysates/proteins at 20 ug per lane.

UGT8 antibody

CATALOG NUMBER: 55-954

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This UGT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-393 amino acids from the Central region of human UGT8.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
61 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UGT8
Additional Names:
2-hydroxyacylsphingosine 1-beta-galactosyltransferase, Ceramide UDP-galactosyltransferase, Cerebroside synthase, UDP-galactose-ceramide galactosyltransferase, UGT8, CGT, UGT4
Protein Accession Number:
Q16880
Protein Gi Number:
296434442
Ncbi Gene Id Number:
7368
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Galactocerebrosides are abundant sphingolipids of themyelin membrane of the central nervous system and peripheralnervous system and are also present in small amounts in kidney. Thekey enzymatic step in the biosynthesis of galactocerebrosidesconsists of the transfer of galactose to ceramide catalyzed byUDP-galactose ceramide galactosyltransferase (CGT, EC 2.4.1.45).The enzyme encoded by the CGT gene is the first involved in complexlipid biosynthesis in the myelinating oligodendrocyte.[supplied byOMIM].
Background Reference 1:
Dziecedil Giel, P., et al. Br. J. Cancer 103(4):524-531(2010)
Background Reference 2:
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Background Reference 3:
Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009)
Background Reference 4:
Ruckhaberle, E., et al. J. Cancer Res. Clin. Oncol. 135(8):1005-1013(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-954

List Size:
400 ul

List Price:

$495.00

Datasheet
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