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Western blot analysis of UHRF1BP1 in mouse kidney tissue lysate with UHRF1BP1 antibody at (A) 1 and (B) 2 μg/mL.
<strong>WB Validation in Human MCF -7 Cells</strong><br>Loading: 15 μg of human tissue lysate Antibodies: UHRF1BP1, 6459, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 1 min
Immunohistochemistry of UHRF1BP1 in human kidney tissue with UHRF1BP1 antibody at 2.5 μg/mL.
Immunofluorescence of UHRF1BP1 in human kidney tissue with UHRF1BP1 antibody at 20 μg/mL.
Western blot analysis of UHRF1BP1 in mouse kidney tissue lysate with UHRF1BP1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

UHRF1BP1 Antibody

CATALOG NUMBER: 6459

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
UHRF1BP1 antibody was raised against a 17 amino acid synthetic peptide near the center of human UHRF1BP1.
The immunogen is located within amino acids 890 - 940 of UHRF1BP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
UHRF1BP1 antibody can be used for detection of UHRF1BP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
UHRF1BP1 antibody is predicted to not cross-react with UHRF1BP1L
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
UHRF1BP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
UHRF1BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
UHRF1BP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UHRF1BP1
Additional Names:
UHRF1BP1 Antibody: ICBP90, C6orf107, dJ349A12.1, UHRF1-binding protein 1, ICBP90-binding protein 1
Protein Accession Number:
NP_060224
Protein Gi Number:
134152667
Ncbi Gene Id Number:
54887
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
UHRF1BP1 Antibody: The Ubiquitin-like containing PHD and RING finger domains 1-binding protein 1 (UHRF1BP1), also known as ICBP90, is a transcription and cell cycle regulator. It specifically binds to the histone H3 N-terminal tail when methylated on K9 by two functional domains, a PHD finger that defines the binding specificity and an SRA (SET- and RING-associated) domain that promotes binding activity. UHRF1BP1 is required for proper heterochromatin formation in mammalian cells. Furthermore, UHRF1BP1 is thought to be a pivotal target for the ERK1/2 signaling pathway to control the proliferation of Jurkat T cells.
Background Reference 1:
Hopfner R, Mousli M, Jeltsch JM, et al. ICBP90, a novel human CCAAT binding protein, involved in the regulation of Topoisomerase IIα expression. Cancer Res. 2000; 60:121-8
Background Reference 2:
Unoki M, Nishidate T and Nakamura Y. ICBP90, an E2F-1 target, recruits HDAC1 and binds to methyl-CpG through its SRA domain. Oncogene 2004; 23:7601-10.
Background Reference 3:
Karagianni P, Amazit L, Qin J, et al. ICBP90, a novel methyl K9 H3 binding protein linking protein ubiquitination with heterochromatin formation. Mol. Cell Biol. 2008; 28:705-17.
Background Reference 4:
Fang Z, Xing F, Bronner C, et al. ICBP90 mediates the ERK1/2 signaling to regulate the proliferation of Jurkat T cells. Cell Immunol. 2009; 257:80-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6459

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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