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Western blot analysis of ULK4 in 3T3 cell lysate with ULK4 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of ULK4 in human brain tissue with ULK4 antibody at 5 μg/mL.
Immunofluorescence of ULK4 in human brain tissue with ULK4 antibody at 20 μg/mL.
Western blot analysis of ULK4 in 3T3 cell lysate with ULK4 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

ULK4 Antibody

CATALOG NUMBER: 7587

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
ULK4 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human ULK4.
The immunogen is located within amino acids 1120 - 1170 of ULK4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ULK4 antibody can be used for detection of ULK4 by Western blot at 0.5 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
ULK4 antibody is human and mouse reactive. Multiple isoforms of ULK4 are known to exist.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 140 kDa
Observed: 165 kDa
Purification:
ULK4 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ULK4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ULK4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
ULK4
Additional Names:
ULK4 Antibody: FAM7C1, REC01035Unc-51-like kinase 4
Protein Accession Number:
NP_060356
Protein Gi Number:
151301204
Ncbi Gene Id Number:
54986
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ULK4 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1). Knockout of ULK genes results in a severe defect in the autophagy pathway (2). ULK4, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK3, is highly conserved among eukaryotes (3). ULK4 has been implicated as a gene involved in the development of hydrocephalus (3). This neurologic disorder is being used in animal models to elucidate factors responsible for the excessive accumulation of cerebrospinal fluid in hydrocephalic humans (4).
Background Reference 1:
Suzuki K, Kubota Y, Sekito T, et al. Hierarchy of Atg proteins in pre-autophagosomal structure organization. Genes to Cells 2007; 12:209–18.
Background Reference 2:
Lee EJ and Tournier C. The requirement of uncoordinated 51-like kinase 1 (ULK1) and ULK2 in the regulation of autophagy. Autophagy 2011; 7:689-95.
Background Reference 3:
Zhou X, Babu JR, da Silva S, et al. Unc-51-like kinase 1/2-mediated endocytic processes regulate filopodia extension and branching of sensory axons. Proc. Natl. Acad. Sci. USA 2007; 104:5842-7.
Background Reference 4:
Vogel P, Read RW, Hansen GM, et al. Congenital hydrocephalus in genetically engineered mice. Vet. Pathol. 2012; 49:166-81.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7587

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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