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Western blot analysis of UNC13B Antibody in 293 cell line lysates (35ug/lane)
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of UNC13B Antibody in 293 cell line lysates (35ug/lane)

UNC13B Antibody

CATALOG NUMBER: 63-695

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This UNC13B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1062-1091 amino acids from the Central region of human UNC13B.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
181 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UNC13B
Additional Names:
Protein unc-13 homolog B, Munc13-2, munc13, UNC13B, UNC13
Protein Accession Number:
O14795
Ncbi Gene Id Number:
10497
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
UNC13B plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. It is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses (By similarity).
Background Reference 1:
Sjoeblom T., et.al., Science 314:268-274(2006).
Background Reference 2:
Yu L.-R., Zhu Z., et.al., J. Proteome Res. 6:4150-4162(2007).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-695

List Size:
400 ul

List Price:

$495.00

Datasheet
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