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46-552 (2ug/ml) staining of Human T-lymphocyte lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-552 (2.5ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-552 (0.5ug/ml) staining of paraffin embedded Mouse Lung (wt in A and KO in B) .
46-552 (2ug/ml) staining of Human T-lymphocyte lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

UNC13D Antibody

CATALOG NUMBER: 46-552

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-ETQKHKKDLHPLFD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx. 105kDa band observed in Human T-lymphocyte and HeLa lysates (calculated MW of 123kDa according to NP_954712.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Thymus shows cytoplasm staining in select areas. Recommended concentration: 2.5ug/ml.In paraffin embedded Mouse Lung shows staining of the resperatory epithelium in wildtype but not in the knock-out. Data kindly provided by Micheal Tuvim, University of Texas MD Anderson Cancer Center, Houston, USA.Recommended concentration: 1-2ug/ml.
Positive Control 1:
Human T-Lymphocyte Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
UNC13D
Additional Names:
UNC13D, Munc13-4, unc-13 homolog D (C. elegans) , FHL3, HLH3, HPLH3, unc-13 homolog D
Protein Accession Number:
NP_954712.1
Protein Gi Number:
46195765
Ncbi Gene Id Number:
201294
Background Reference 1:
Zur Stadt U, Beutel K, Kolberg S, Schneppenheim R, Kabisch H, Janka G, Hennies HC. Mutation spectrum in children with primary hemophagocytic lymphohistiocytosis: molecular and functional analyses of PRF1, UNC13D, STX11, and RAB27A. Hum Mutat. 2006 Jan;27(1):62-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-552

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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