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43-468 (2ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
43-468 (2ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

UNC5B Antibody

CATALOG NUMBER: 43-468

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SARDWIFQLKTQAHQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 90kDa band observed in Human Bone Marrow lysates (calculated MW of 104kDa according to NP_734465.2). Some minor background is detected and is blocked by the immunizing peptide. We call for caution when used for other assays than Western blot. Recommended concentration: 1-3ug/ml.
Predicted Molecular Weight:
Approx 90kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
UNC5B
Additional Names:
UNC5B, unc-5 homolog B (C. elegans), UNC5H2, p53RDL1, netrin receptor UNC5B, p53-regulated receptor for death and life protein 1, protein unc-5 homolog 2, protein unc-5 homolog B, transmembrane receptor Unc5H2, unc-5 homolog 2
Protein Accession Number:
NP_734465.2
Protein Gi Number:
85861262
Ncbi Gene Id Number:
219699
Background Reference 1:
Tang X, Jang SW, Okada M, Chan CB, Feng Y, Liu Y, Luo SW, Hong Y, Rama N, Xiong WC, Mehlen P, Ye K.Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-468

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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