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<strong>Figure 1 Western Blot Validation in Mouse C2C12 Cell Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: UNG1, 3865 (A: 0.5 μg/mL, B: 1 μg/mL and C: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: UNG1, 3863 (0.5 μg/mL), UNG1, 3865 (4 μg/mL), beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Immunohistochemistry Validation of UNG1 in Human Heart Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-UNG1 antibody (3865) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 4 Immunofluorescence Validation of UNG1 in Human Heart Tissue</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed human heart labeling UNG1 with 3865 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Figure 1 Western Blot Validation in Mouse C2C12 Cell Lysate
Loading: 15 μg of lysates per lane.Antibodies: UNG1, 3865 (A: 0.5 μg/mL, B: 1 μg/mL and C: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
IAV
Trial Size

UNG1 Antibody

CATALOG NUMBER: 3865

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat (100%).
Immunogen:
Anti-UNG1 antibody (3865) was raised against a peptide corresponding to 14 amino acids near the center of human UNG1.
The immunogen is located within amino acids 200-250 of UNG1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WB: 0.5-4 μg/mL; IHC: 2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1285 - C2C12 Cell Lysate
Positive Control 2:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 3:
Cat. No. 10-501 - Human Heart Tissue Slide
Predicted Molecular Weight:
Predicted: 34kD
Observed: 34 kD
Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar UNG1 expression profile in human cell lines detected by two independent anti-UNG1 antibodies that recognize different epitopes, 3863 against C-terminus domain and 3865 against central domain.  UNG1 proteins are detected in all tested cell lines at different expression levels by the two independent antibodies. 

Isoforms:
Human UNG1 has 2 isoforms, including isoform 1 (304aa, 34kD) and isoform 2 (313aa, 35kD). Mouse UNG1 has 2 isoforms, including isoform 1 (295aa, 33kD) and isoform 2 (306aa, 34kD). Rat UNG1 has one isoform (303aa, 34kD). 3865 can detect human, mouse and rat.
Purification:
UNG1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
UNG1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
UNG1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UNG
Additional Names:
UNG1 Antibody: DGU, UDG, UNG1, UNG2, HIGM4, HIGM5, UNG15, DGU, Uracil-DNA glycosylase
Protein Accession Number:
NP_003353
Protein Gi Number:
6224979
Ncbi Gene Id Number:
7374
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
UNG1 Antibody: The human uracil-DNA glycosylase (UNG) gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms through differentially regulated promotes and alternative splicing. While UNG2 is the major enzyme in the base excision repair pathway that removes uracil residues from nuclear DNA that arise through either misincorporation during replication or cytosine deamination, inhibition of UNG1 by uracil glycosylase inhibitor did not lead to increased levels of spontaneous or induced mitochondrial DNA mutations. However, decreased levels of UNG activity and increased oxidative damage to mitochondrial DNA were seen in older mice, suggesting that mitochondrial DNA repair mechanisms may be involved in various neurodegenerative disorders in an age-dependent manner. This UNG1 antibody will not cross-react with UNG2.
Background Reference 1:
Krokan et al. Prog. Nucleic Acid Res. Mol. Biol. 2001; 68:365-86.
Background Reference 2:
Krokan et al. Prog. Nucleic Acid Res. Mol. Biol. 2001; 68:365-86.
Background Reference 3:
Kachhap and Singh. Mol. Cancer 2004; 3:32.
Background Reference 4:
Imam et al. Neurobiol. Aging 2006; 27:1129-36.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3865

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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