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Western blot analysis of UPK1B in human kidney tissue lysate with UPK1B antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Immunohistochemistry of UPK1B in human bladder tissue with UPK1B antibody at 5 μg/mL.
Immunofluorescence of UPK1B in human bladder muscle tissue with UPK1B antibody at 20 μg/mL.
Western blot analysis of UPK1B in human kidney tissue lysate with UPK1B antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Trial Size

UPK1B Antibody

CATALOG NUMBER: 6499

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
UPK1B antibody was raised against a 17 amino acid synthetic peptide near the center of human UPK1B.
The immunogen is located within amino acids 110 - 160 of UPK1B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
UPK1B antibody can be used for detection of UPK1B by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of UPK1B are known to exist; this antibody will recognize both isoforms. UPK1B antibody is predicted to not cross-react with other tetraspanin protein family members.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Predicted Molecular Weight:
Predicted: 29 kDa
Observed: 30 kDa
Purification:
UPK1B Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
UPK1B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
UPK1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UPK1B
Additional Names:
UPK1B Antibody: UPIB, UPK1, TSPAN20, Uroplakin-1b, Tetraspanin-20, UP1b
Protein Accession Number:
NP_008883
Protein Gi Number:
49619237
Ncbi Gene Id Number:
7348
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
UPK1B Antibody: Uroplakin-1B (UPK1B) is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. UPK1B is found in the asymmetrical unit membrane (AUM) where it can form a complex with other transmembrane 4 superfamily proteins and may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.
Background Reference 1:
Yu J, Lin JH, Wu XR, et al. Uroplakins Ia and Ib, two major differentiation products of bladder epithelium, belong to a family of four transmembrane domain (4TM) proteins. J. Cell Biol. 1994; 125:171-82.
Background Reference 2:
Wu XR, Kong XP, Pellicer A, et al. Uroplakins in urothelial biology, function, and disease. Kidney Int. 2009; 75:1153-65
Background Reference 3:
Sun TT, Zhao H, Provet J, et al. Formation of asymmetric unit membrane during urothelial differentiation. Mol. Biol. Rep. 1996; 23:3-11.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6499

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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