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Western blot analysis of extracts of various cell lines, using UQCRC1 antibody (19-039) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat brain using UQCRC1 antibody (19-039) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using UQCRC1 antibody (19-039) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using UQCRC1 antibody (19-039) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using UQCRC1 antibody (19-039) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

UQCRC1 Antibody

CATALOG NUMBER: 19-039

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 156-480 of human UQCRC1 (NP_003356.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
A-549
Positive Control 2:
HepG2
Positive Control 3:
HeLa
Positive Control 4:
293T
Positive Control 5:
COS-7
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 53kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
UQCRC1
Additional Names:
Cytochrome b-c1 complex subunit 1, mitochondrial, Complex III subunit 1, Core protein I, Ubiquinol-cytochrome-c reductase complex core protein 1, UQCRC1
Ncbi Gene Id Number:
7384
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-039

List Size:
100 uL

List Price:

$436.00

Datasheet
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