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Western blot analysis of extracts of various cell lines, using UQCRC2 antibody (19-301) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human liver damage using UQCRC2 antibody (19-301) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug UQCRC2 antibody (19-301). Western blot was performed from the immunoprecipitate using UQCRC2 antibody (19-301) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using UQCRC2 antibody (19-301) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

UQCRC2 Antibody

CATALOG NUMBER: 19-301

Clonality:
Polyclonal
Tested Applications:
IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 224-453 of human UQCRC2 (NP_003357.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IP: 1:50 - 1:200
Positive Control 1:
U-251MG
Positive Control 2:
MCF7
Positive Control 3:
HepG2
Positive Control 4:
H460
Positive Control 5:
Mouse kidney
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 48kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
UQCRC2
Additional Names:
Cytochrome b-c1 complex subunit 2, mitochondrial, Complex III subunit 2, Core protein II, Ubiquinol-cytochrome-c reductase complex core protein 2, UQCRC2
Ncbi Gene Id Number:
7385
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is located in the mitochondrion, where it is part of the ubiquinol-cytochrome c reductase complex (also known as complex III). This complex constitutes a part of the mitochondrial respiratory chain. Defects in this gene are a cause of mitochondrial complex III deficiency nuclear type 5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-301

List Size:
100 uL

List Price:

$436.00

Datasheet
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