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43-218 (0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-218 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-218 (0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UROD Antibody

CATALOG NUMBER: 43-218

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: KARECVGKTVTLQGN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in lysates of cell line K56 (calculated MW of 40.8kDa according to NP_000365.3). Recommended concentration: 0.1-0.3ug/ml.An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Predicted Molecular Weight:
Approx 40kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
UROD
Additional Names:
UROD, uroporphyrinogen decarboxylase, PCT, UPD, OTTHUMP00000010502, OTTHUMP00000010503, uroporphyrinogen III decarboxylase
Protein Accession Number:
NP_000365.3
Protein Gi Number:
71051616
Ncbi Gene Id Number:
7389
Background Reference 1:
Darwich E, To-Figueras J, Badenas C, Herrero C.Hepatoerythropoietic porphyria and familial porphyria cutanea tarda in Spanish patients: G281E mutation in the uroporphyrinogen decarboxylase gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-218

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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