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46-554 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-554 (10ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. (Data have been obtained with 46-554 from a previous batch).
46-554 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

USH1C Antibody

CATALOG NUMBER: 46-554

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: DRKVAREFRHKVD-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75kDa band observed in lysates of cell line HEK293 (calculated MW of 62.2kDa according to NP_005700.2). Recommended concentration: 0.1-0.3ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_005700.2 and NP_710142.1).
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
USH1C
Additional Names:
USH1C, harmonin, AIE-75, DFNB18, PDZ-45, PDZ-73, HARMONIN, NY-CO-37, NY-CO-38, PDZ-73/NY-CO-38, Usher syndrome 1C (autosomal recessive, severe), PDZ-73 protein, ush1cpst, deafness, autosomal recessive 18
Protein Accession Number:
NP_005700.2,NP_710142.1
Protein Gi Number:
71480164
Ncbi Gene Id Number:
10083
Background Reference 1:
Verpy E, Leibovici M, Zwaenepoel I, Liu XZ, Gal A, Salem N, Mansour A, Blanchard S, Kobayashi I, Keats BJ, Slim R, Petit C. A defect in harmonin, a PDZ domain-containing protein expressed in the inner ear sensory hair cells, underlies Usher syndrome type 1C. Nat Genet. 2000 Sep;26(1):51-5.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-554

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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