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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
USO1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).

USO1 Antibody

CATALOG NUMBER: 57-431

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This USO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 932-960 amino acids from the C-terminal region of human USO1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
108 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
USO1
Additional Names:
General vesicular transport factor p115, Protein USO1 homolog, Transcytosis-associated protein, TAP, Vesicle-docking protein, USO1, VDP
Protein Accession Number:
O60763
Ncbi Gene Id Number:
8615
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a peripheral membraneprotein which recycles between the cytosol and the Golgi apparatusduring interphase. It is regulated by phosphorylation:dephosphorylated protein associates with the Golgi membrane anddissociates from the membrane upon phosphorylation. Ras-associatedprotein 1 recruits this protein to coat protein complex II (COPII)vesicles during budding from the endoplasmic reticulum, where itinteracts with a set of COPII vesicle-associated SNAREs to form acis-SNARE complex that promotes targeting to the Golgi apparatus.Transport from the ER to the cis/medial Golgi compartments requiresthe action of this gene product, GM130 and giantin in a sequentialmanner.
Background Reference 1:
Striegl, H., et al. PLoS ONE(2), E8991 (2010) :
Background Reference 2:
Merk, M., et al. J. Immunol. 182(11):6896-6906(2009)
Background Reference 3:
Mukherjee, S., et al. J. Biol. Chem. 284(3):1709-1717(2009)
Background Reference 4:
Striegl, H., et al. PLoS ONE(2), E4656 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-431

List Size:
400 ul

List Price:

$495.00

Datasheet
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