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Western blot analysis of anti-USP1 Pab in HepG2 cell line lysate (35ug/lane).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of anti-USP1 Pab in HepG2 cell line lysate (35ug/lane).

USP1 Antibody

CATALOG NUMBER: 61-091

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This USP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 607-637 amino acids from the C-terminal region of human USP1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
88 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
USP1
Additional Names:
Ubiquitin carboxyl-terminal hydrolase 1, Deubiquitinating enzyme 1, hUBP, Ubiquitin thioesterase 1, Ubiquitin-specific-processing protease 1, USP1
Protein Accession Number:
O94782
Ncbi Gene Id Number:
7398
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Deubiquitinating enzymes (DUBs) are capable of producing a free ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins in cells. DUBs possess conserved catalytic regions known as the 'cys domain' and the 'his domain.' There are 2 distinct families of DUBs: ubiquitin-specific processing proteases (UBPs, or USPs) and ubiquitin C-terminal hydrolases (UCHs).
Background Reference 1:
Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003).
Background Reference 2:
Fujiwara, T., et al., Genomics 54(1):155-158 (1998).
Background Reference 3:
D'Andrea, A., et al., Crit. Rev. Biochem. Mol. Biol. 33(5):337-352 (1998).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-091

List Size:
400 ul

List Price:

$495.00

Datasheet
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