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Western blot analysis of USP10 in Jurkat cell lysate with USP10 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of USP10 in Jurkat cells with USP10 antibody at 20 μg/mL.
Immunofluorescence of USP10 in Jurkat cells with USP10 antibody at 20 μg/mL.
Western blot analysis of USP10 in Jurkat cell lysate with USP10 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

USP10 Antibody

CATALOG NUMBER: 5803

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (80%), Rat: (91%), Chicken: (89%)
Immunogen:
USP10 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human USP10.
The immunogen is located within amino acids 30 - 80 of USP10.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
USP10 antibody can be used for detection of USP10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 17-005 - Jurkat Cell Slide
Purification:
USP10 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
USP10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
USP10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
USP10
Additional Names:
USP10 Antibody: UBPO, KIAA0190, Ubiquitin carboxyl-terminal hydrolase 10, Deubiquitinating enzyme 10
Protein Accession Number:
NP_005144
Protein Gi Number:
119220605
Ncbi Gene Id Number:
9100
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
USP10 Antibody: USP10, also known as ubiquitin specific peptidase 10, belongs to the ubiquitin-specific protease family of cysteine proteases. USP10 functions to catalyze the cleavage of ubiquitin from ubiquitin-conjugated protein substrates such as p53/TP53, SNX3 and CFTR. USP10 has been identified as a subunit of DNA-bound androgen receptor (AR) complexes and may play a role in the activity of the DNA-bound androgen receptor complex. USP10 also acts as an essential regulator of p53/TP53 stability and is thought to function as a tumor suppressor.
Background Reference 1:
D'Andrea A and Pellman D. Deubiquitinating enzymes: a new class of biological regulators. Crit. Rev. Biochem. Mol. Biol.1998; 33:337-52.
Background Reference 2:
Bomberger JM, Barnaby RL, and Stanton BA. The deubiquitinating enzyme USP10 regulates the endocytic recycling of CFTR in airway epithelial cells. Channels2010; 4(3).
Background Reference 3:
Faus H, Meyer HA, Huber M, et al. The ubiquitin-specific protease USP10 modulates androgen receptor function. Mol. Cell. Endocrinol.2005; 245:138-46.
Background Reference 4:
Yuan J, Luo K, Zhang L, et al. USP10 regulates p53 localization and stability by deubiquitinating p53. Cell 2010; 140:384-96.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5803

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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