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46-555 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
46-555 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

USP11 Antibody

CATALOG NUMBER: 46-555

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: CSSPPSSEFMDVN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 120kDa band observed in lysates of cell line Jurkat (calculated MW of 110kDa according to NP_004642.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ideguchi et al, Biochem J. 2002 Oct 1;367(Pt 1):87-95.; PMID: 12084015). Recommended concentration: 1-3ug/ml.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
USP11
Additional Names:
USP11, UHX1, ubiquitin specific protease 11, deubiquitinating enzyme 11, ubiquitin thiolesterase 11, ubiquitin carboxyl-terminal hydrolase, X-linked, ubiquitin specific peptidase 11, RP4-659F15.2, ubiquitin-specific processing protease 11
Protein Accession Number:
NP_004642.2
Protein Gi Number:
24234683
Ncbi Gene Id Number:
8237
Background Reference 1:
Ideguchi H, Ueda A, Tanaka M, Yang J, Tsuji T, Ohno S, Hagiwara E, Aoki A, Ishigatsubo Y. Structural and functional characterization of the USP11 deubiquitinating enzyme, which interacts with the RanGTP-associated protein RanBPM. Biochem J. 2002 Oct 1;367(Pt 1):87-95.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-555

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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