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USP16 Antibody

CATALOG NUMBER: 42-222

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Horse, Dog, Cow
Immunogen:
The immunogen for this antibody is: NVEWNICQDCKTDNK
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 50kDa and 26kDa in lysates of cell lines HEK293, A549 and HeLa after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.5kDa according to NP_006438.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
Reported variants represent identical protein: NP_001027582.1 NP_006438.1
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
USP16
Additional Names:
USP16, ubiquitin specific peptidase 16, UBP-M, deubiquitinating enzyme 16, ubiquitin carboxyl-terminal hydrolase 16, ubiquitin specific protease 16, ubiquitin thiolesterase 16, ubiquitin-specific processing protease 16
Protein Accession Number:
NP_001027582.1,NP_001001992.1
Protein Gi Number:
75992941
Ncbi Gene Id Number:
10600
Background Reference 1:
Joo HY, Zhai L, Yang C, Nie S, Erdjument-Bromage H, Tempst P, Chang C, Wang H Regulation of cell cycle progression and gene expression by H2A deubiquitination Nature. 2007 Oct 25;449(7165):1068-72. Epub 2007 Oct 3

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-222

List Size:
0.1 mg

List Price:

$391.00

Datasheet
Shipping Info

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