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46-558 (3ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
46-558 (1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-558 (3ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

USP20 Antibody

CATALOG NUMBER: 46-558

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-HGEQKIEAETRAV
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 100kDa band observed in Human Tonsil lysates (calculated MW of 102kDa according to NP_006667.3). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Lymph Node shows strongest staining in smooth muscle cells surrounding a vessel. Data obtained from a previous batch (different goat). Recommended concentration, 3 ug/ml.
Specificity:
Reported variants represent identical protein (NP_006667.3; NP_001103773.2; NP_001008563.2).
Positive Control 1:
Cat. No. 1369 - Human Lymph Node Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
USP20
Additional Names:
USP20, VDU2, ubiquitin specific protease 20, KIAA1003, pVHL-interacting deubiquitinating enzyme 2, ubiquitin specific peptidase 20, LSFR3A
Protein Accession Number:
NP_006667.3
Protein Gi Number:
183397134
Ncbi Gene Id Number:
10868
Background Reference 1:
Curcio-Morelli C, Zavacki AM, Christofollete M, Gereben B, de Freitas BC, Harney JW, Li Z, Wu G, Bianco AC. Deubiquitination of type 2 iodothyronine deiodinase by von Hippel-Lindau protein-interacting deubiquitinating enzymes regulates thyroid hormone activation. J Clin Invest. 2003 Jul;112(2):189-96.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-558

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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