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Western blot analysis of USP25 in mouse brain tissue lysate with USP25 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of USP25 in human brain tissue with USP25 antibody at 5 μg/mL.
Immunofluorescence of USP25 in human brain tissue with USP25 antibody at 20 μg/mL.
Western blot analysis of USP25 in mouse brain tissue lysate with USP25 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

USP25 Antibody

CATALOG NUMBER: 7765

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25.
The immunogen is located within amino acids 470 - 520 of USP25.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
USP25 antibody can be used for detection of USP25 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 116 kDa
Observed: 105 kDa
Purification:
USP25 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
USP25 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
USP25 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
USP25
Additional Names:
USP25 Antibody: USP21, USP21, Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25
Protein Accession Number:
NP_037528
Protein Gi Number:
50312666
Ncbi Gene Id Number:
29761
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
USP25 (ubiquitin specific peptidase 25), also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response (1). It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform (2). The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function. USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling (3,4).
Background Reference 1:
Valero R, Marfany G, Gonzalez-Angulo O, et al. USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2. Genomics 1999; 62:395-405.
Background Reference 2:
Valero R, Bayes M, Francisca Sanchez-Font M, et al. Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25. Genome Biol. 2001; 2: RESEARCH0043.
Background Reference 3:
Zhong B, Liu X, Wang X, et al. Negative regulation of IL-17-mediated signaling and inflammation by the ubiquitin-specific protease USP25. Nat. Immunol. 2012; 13:1110-7.
Background Reference 4:
Zhong B, Liu X, Wang X, et al. Ubiquitin-specific protease 25 regulates TLR4-dependent innate immune responses through deubiquitination of the adaptor protein TRAF3. Sci. Signal. 2013; 6:ra35.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7765

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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