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Western blot analysis of various lysates, using USP25 Rabbit mAb (A23431) at 1:5000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using USP25 Rabbit mAb (A23431) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using USP25 Rabbit mAb (A23431) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunoprecipitation of USP25 from 300 µg extracts of 293F cells was performed using 0.5 µg of USP25 Rabbit mAb (A23431). Rabbit IgG isotype control (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using USP25 Rabbit mAb (A23431) at a dilution of 1:5000.
Immunoprecipitation of USP25 from 300 µg extracts of Mouse testis was performed using 0.5 µg of USP25 Rabbit mAb (A23431). Rabbit IgG isotype control (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using USP25 Rabbit mAb (A23431) at a dilution of 1:5000.
Western blot analysis of various lysates, using USP25 Rabbit mAb (A23431) at 1:5000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 60s.

USP25 Rabbit Recombinant Antibody

CATALOG NUMBER: 82-602

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC-P, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, IP, WB
Application Note:
WB,1:2000 - 1:60000, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, IHC-P,1:50 - 1:20, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
122KDa/125KDa/129KDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
USP25
Additional Names:
USP21; USP25
Protein Accession Number:
Q9UHP3
Ncbi Gene Id Number:
29761
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-602

List Size:
100 uL

List Price:

$540.00

Datasheet
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